• Western blot analysis of extracts of MCF-7 cells, using CHD5 antibody (STJ113575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-CHD5 antibody (1530-1700) (STJ113575)

SKU:
STJ113575

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHD5 (1530-1700) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD5
Gene ID: 26038
Uniprot ID: CHD5_HUMAN
Immunogen Region: 1530-1700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1530-1700 of human CHD5 (NP_056372.1).
Immunogen Sequence: IPEGPEGKKSGEVISSDPNT PVPASPAHLLPAPLGLPDKM EAQLGYMDEKDPGAQKPRQP LEVQALPAALDRVESEDKHE SPASKERAREERPEETEKAP PSPEQLPREEVLPEKEKILD KLELSLIHSRGDSSELRPDD TKAEEKEPIETQQNGDKEED DEGKKEDKKGK
Tissue Specificity Preferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland and detected in testis.
Post Translational Modifications Methylated at Gln-1390 by N6AMT1.
Function Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 5
Chd-5
Atp-Dependent Helicase Chd5
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 5 antibody
Anti-Chd-5 antibody
Anti-Atp-Dependent Helicase Chd5 antibody
Anti-CHD5 antibody
Anti-KIAA0444 antibody

Information sourced from Uniprot.org

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