| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CHKA (1-170) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHKA |
| Gene ID: |
1119 |
| Uniprot ID: |
CHKA_HUMAN |
| Immunogen Region: |
1-170 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CHKA (NP_001268.2). |
| Immunogen Sequence: |
MKTKFCTGGEAEPSPLGLLL SCGSGSAAPAPGVGQQRDAA SDLESKQLGGQQPPLALPPP PPLPLPLPLPQPPPPQPPAD EQPEPRTRRRAYLWCKEFLP GAWRGLREDEFHISVIRGGL SNMLFQCSLPDTTATLGDEP RKVLLRLYGAILQMRSCNKE GSEQAQKENE |
| Post Translational Modifications | Isoform 1: Phosphorylated at Ser-279 by AMPK in response to glucose deprivation, leading to localization to lipid droplets. Isoform 1: Acetylated by KAT5 at Lys-247 following phosphorylation by AMPK, leading to monomerization and conversion into a tyrosine-protein kinase. |
| Function | Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis. Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis. Has higher activity with choline. May contribute to tumor cell growth. Isoform 1: This isoform plays a key role in lipolysis of lipid droplets following glucose deprivation. In response to glucose deprivation, phosphorylated by AMPK, promoting localization to lipid droplets. Phosphorylation is followed by acetylation by KAT5, leading to dissociation of the homodimer into a monomer. Monomeric CHKA isoform 1 is converted into a tyrosine-protein kinase, which phosphorylates lipid droplet structural proteins PLIN2 and PLIN3, leading to lipolysis of lipid droplets. |
| Protein Name | Choline Kinase AlphaCkChetk-AlphaEthanolamine KinaseEk |
| Database Links | Reactome: R-HSA-1483191Reactome: R-HSA-1483213 |
| Cellular Localisation | CytoplasmCytosolIsoform 1: Lipid DropletIsoform 1 Localizes To Lipid Droplets Following Phosphorylation By Ampk |
| Alternative Antibody Names | Anti-Choline Kinase Alpha antibodyAnti-Ck antibodyAnti-Chetk-Alpha antibodyAnti-Ethanolamine Kinase antibodyAnti-Ek antibodyAnti-CHKA antibodyAnti-CHK antibodyAnti-CKI antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
