• Western blot analysis of extracts of rat skeletal muscle, using CHRNA2 antibody (STJ112096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CHRNA2 antibody (27-264) (STJ112096)

SKU:
STJ112096

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRNA2 (27-264) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRNA2
Gene ID: 1135
Uniprot ID: ACHA2_HUMAN
Immunogen Region: 27-264
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-264 of human CHRNA2 (NP_000733.2).
Immunogen Sequence: EEAKRPPPRAPGDPLSSPSP TALPQGGSHTETEDRLFKHL FRGYNRWARPVPNTSDVVIV RFGLSIAQLIDVDEKNQMMT TNVWLKQEWSDYKLRWNPTD FGNITSLRVPSEMIWIPDIV LYNNADGEFAVTHMTKAHLF STGTVHWVPPAIYKSSCSID VTFFPFDQQNCKMKFGSWTY DKAKIDLEQMEQTVDLKDYW ESGEWAIVNATGTYNSKKYD CCAEIYPDVTYAFVIRRL
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Protein Name Neuronal Acetylcholine Receptor Subunit Alpha-2
Database Links Reactome: R-HSA-629594
Reactome: R-HSA-629597
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Neuronal Acetylcholine Receptor Subunit Alpha-2 antibody
Anti-CHRNA2 antibody

Information sourced from Uniprot.org

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