• Western blot analysis of extracts of various cell lines, using CHRNA5 antibody (STJ23135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CHRNA5 antibody (23-240) (STJ23135)

SKU:
STJ23135

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRNA5 (23-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRNA5
Gene ID: 1138
Uniprot ID: ACHA5_HUMAN
Immunogen Region: 23-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-240 of human CHRNA5 (NP_000736.2).
Immunogen Sequence: RCGLAGAAGGAQRGLSEPSS IAKHEDSLLKDLFQDYERWV RPVEHLNDKIKIKFGLAISQ LVDVDEKNQLMTTNVWLKQE WIDVKLRWNPDDYGGIKVIR VPSDSVWTPDIVLFDNADGR FEGTSTKTVIRYNGTVTWTP PANYKSSCTIDVTFFPFDLQ NCSMKFGSWTYDGSQVDIIL EDQDVDKRDFFDNGEWEIVS ATGSKGNRTDSCCWYPYV
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Protein Name Neuronal Acetylcholine Receptor Subunit Alpha-5
Database Links Reactome: R-HSA-629594
Reactome: R-HSA-629597
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Neuronal Acetylcholine Receptor Subunit Alpha-5 antibody
Anti-CHRNA5 antibody
Anti-NACHRA5 antibody

Information sourced from Uniprot.org

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