• Western blot analysis of extracts of various cell lines, using CHRNB3 antibody (STJ112147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CHRNB3 antibody (25-232) (STJ112147)

SKU:
STJ112147

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRNB3 (25-232) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRNB3
Gene ID: 1142
Uniprot ID: ACHB3_HUMAN
Immunogen Region: 25-232
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-232 of human CHRNB3 (NP_000740.1).
Immunogen Sequence: IAENEDALLRHLFQGYQKWV RPVLHSNDTIKVYFGLKISQ LVDVDEKNQLMTTNVWLKQE WTDHKLRWNPDDYGGIHSIK VPSESLWLPDIVLFENADGR FEGSLMTKVIVKSNGTVVWT PPASYKSSCTMDVTFFPFDR QNCSMKFGSWTYDGTMVDLI LINENVDRKDFFDNGEWEIL NAKGMKGNRRDGVYSYPFIT YSFVLRRL
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Protein Name Neuronal Acetylcholine Receptor Subunit Beta-3
Database Links Reactome: R-HSA-629594
Reactome: R-HSA-629597
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Neuronal Acetylcholine Receptor Subunit Beta-3 antibody
Anti-CHRNB3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance