• Western blot analysis of extracts of various cell lines, using Creatine kinase B type (Creatine kinase B type (CKB) ) antibody (STJ114505) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CKB antibody (182-381) (STJ114505)

SKU:
STJ114505

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CKB (182-381) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CKB
Gene ID: 1152
Uniprot ID: KCRB_HUMAN
Immunogen Region: 182-381
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 182-381 of human Creatine kinase B type (Creatine kinase B type (CKB) ) (NP_001814.2).
Immunogen Sequence: AEQQQLIDDHFLFDKPVSPL LLASGMARDWPDARGIWHND NKTFLVWVNEEDHLRVISMQ KGGNMKEVFTRFCTGLTQIE TLFKSKDYEFMWNPHLGYIL TCPSNLGTGLRAGVHIKLPN LGKHEKFSEVLKRLRLQKRG TGGVDTAAVGGVFDVSNADR LGFSEVELVQMVVDGVKLLI EMEQRLEQGQAIDDLMPAQK
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
Protein Name Creatine Kinase B-Type
Brain Creatine Kinase
B-Ck
Creatine Kinase B Chain
Creatine Phosphokinase B-Type
Cpk-B
Database Links Reactome: R-HSA-71288
Reactome: R-HSA-9696264
Cellular Localisation Cytoplasm
Cytosol
Mitochondrion
Cell Membrane
Localizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region
Alternative Antibody Names Anti-Creatine Kinase B-Type antibody
Anti-Brain Creatine Kinase antibody
Anti-B-Ck antibody
Anti-Creatine Kinase B Chain antibody
Anti-Creatine Phosphokinase B-Type antibody
Anti-Cpk-B antibody
Anti-CKB antibody
Anti-CKBB antibody

Information sourced from Uniprot.org

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