• Immunofluorescence analysis of U2OS cells using CLDN5 Rabbit polyclonal antibody (STJ112245) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CLDN5 Rabbit polyclonal antibody (STJ112245) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using CLDN5 Rabbit polyclonal antibody (STJ112245) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLDN5 antibody (119-218) (STJ112245)

SKU:
STJ112245

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLDN5 (119-218) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLDN5
Gene ID: 7122
Uniprot ID: CLD5_HUMAN
Immunogen Region: 119-218
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 119-218 of human CLDN5 (NP_001349995.1).
Immunogen Sequence: LTGGVLYLFCGLLALVPLCW FANIVVREFYDPSVPVSQKY ELGAALYIGWAATALLMVGG CLLCCGAWVCTGRPDLSFPV KYSAPRRPTATGDYDKKNYV
Function Plays a major role in tight junction-specific obliteration of the intercellular space.
Protein Name Claudin-5
Transmembrane Protein Deleted In Vcfs
Tmdvcf
Database Links Reactome: R-HSA-420029
Reactome: R-HSA-8935964
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Claudin-5 antibody
Anti-Transmembrane Protein Deleted In Vcfs antibody
Anti-Tmdvcf antibody
Anti-CLDN5 antibody
Anti-AWAL antibody
Anti-TMVCF antibody

Information sourced from Uniprot.org

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