• Western blot analysis of extracts of HepG2 cells, using CLEC4M antibody (STJ116481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CLEC4M antibody (71-399) (STJ116481)

SKU:
STJ116481

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLEC4M (71-399) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLEC4M
Gene ID: 10332
Uniprot ID: CLC4M_HUMAN
Immunogen Region: 71-399
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 71-399 of human CLEC4M (NP_055072.3).
Immunogen Sequence: QVSKVPSSLSQEQSEQDAIY QNLTQLKAAVGELSEKSKLQ EIYQELTQLKAAVGELPEKS KLQEIYQELTRLKAAVGELP EKSKLQEIYQELTRLKAAVG ELPEKSKLQEIYQELTRLKA AVGELPEKSKLQEIYQELTE LKAAVGELPEKSKLQEIYQE LTQLKAAVGELPDQSKQQQI YQELTDLKTAFERLCRHCPK DWTFFQGNCYFMSNSQRNWH DSVTACQEVRAQLVVIKTA
Tissue Specificity Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
Function Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. (Microbial infection) Acts as an attachment receptor for Ebolavirus. (Microbial infection) Acts as an attachment receptor for Hepatitis C virus. (Microbial infection) Acts as an attachment receptor for HIV-1. (Microbial infection) Acts as an attachment receptor for Human coronavirus 229E. (Microbial infection) Acts as an attachment receptor for Human cytomegalovirus/HHV-5. (Microbial infection) Acts as an attachment receptor for Influenzavirus. (Microbial infection) Acts as an attachment receptor for SARS-CoV. (Microbial infection) Acts as an attachment receptor for West-nile virus. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus. (Microbial infection) Acts as an attachment receptor for Marburg virus glycoprotein. (Microbial infection) Recognition of M.bovis by dendritic cells may occur partially via this molecule.
Protein Name C-Type Lectin Domain Family 4 Member M
Cd209 Antigen-Like Protein 1
Dc-Sign-Related Protein
Dc-Signr
Dendritic Cell-Specific Icam-3-Grabbing Non-Integrin 2
Dc-Sign2
Liver/Lymph Node-Specific Icam-3-Grabbing Non-Integrin
L-Sign
Cd Antigen Cd299
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type Ii Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Cell Membrane
Isoform 5: Secreted
Isoform 6: Secreted
Isoform 7: Secreted
Isoform 10: Secreted
Alternative Antibody Names Anti-C-Type Lectin Domain Family 4 Member M antibody
Anti-Cd209 Antigen-Like Protein 1 antibody
Anti-Dc-Sign-Related Protein antibody
Anti-Dc-Signr antibody
Anti-Dendritic Cell-Specific Icam-3-Grabbing Non-Integrin 2 antibody
Anti-Dc-Sign2 antibody
Anti-Liver/Lymph Node-Specific Icam-3-Grabbing Non-Integrin antibody
Anti-L-Sign antibody
Anti-Cd Antigen Cd299 antibody
Anti-CLEC4M antibody
Anti-CD209L antibody
Anti-CD209L1 antibody
Anti-CD299 antibody

Information sourced from Uniprot.org

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