• Western blot analysis of extracts of various cell lines, using CLN5 antibody (STJ114752) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human appendix using CLN5 antibody (STJ114752) at dilution of 1:150 (40x lens).

Anti-CLN5 antibody (96-407) (STJ114752)

SKU:
STJ114752

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLN5 (96-407) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLN5
Gene ID: 1203
Uniprot ID: CLN5_HUMAN
Immunogen Region: 96-407
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 96-407 of human CLN5 (NP_006484.1).
Immunogen Sequence: IPSRRHWPVPYKRFDFRPKP DPYCQAKYTFCPTGSPIPVM EGDDDIEVFRLQAPVWEFKY GDLLGHLKIMHDAIGFRSTL TGKNYTMEWYELFQLGNCTF PHLRPEMDAPFWCNQGAACF FEGIDDVHWKENGTLVQVAT ISGNMFNQMAKWVKQDNETG IYYETWNVKASPEKGAETWF DSYDCSKFVLRTFNKLAEFG AEFKNIETNYTRIFLYSGEP TYLGNETSVFGPTGNKTLG
Tissue Specificity Ubiquitous.
Post Translational Modifications N-glycosylated with both high mannose and complex type sugars. Glycosylation is important for proper folding and trafficking to the lysosomes. Ceroid-lipofuscinosis neuronal protein 5: The type II membrane signal anchor is proteolytically cleaved to produce a mature form that is transported to the lysosomes (Ceroid-lipofuscinosis neuronal protein 5, secreted form). Can undergo proteolytic cleavage at the C-terminus, probably by a cysteine protease and may involve the removal of approximately 10-15 residues from the C-terminal end.
Function Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.
Protein Name Ceroid-Lipofuscinosis Neuronal Protein 5
Protein Cln5 Cleaved Into - Ceroid-Lipofuscinosis Neuronal Protein 5 - Secreted Form
Cellular Localisation Ceroid-Lipofuscinosis Neuronal Protein 5
Secreted Form: Lysosome
Ceroid-Lipofuscinosis Neuronal Protein 5: Membrane
Single-Pass Type Ii Membrane Protein
An Amphipathic Anchor Region Facilitates Its Association With The Membrane
Alternative Antibody Names Anti-Ceroid-Lipofuscinosis Neuronal Protein 5 antibody
Anti-Protein Cln5 Cleaved Into - Ceroid-Lipofuscinosis Neuronal Protein 5 - Secreted Form antibody
Anti-CLN5 antibody

Information sourced from Uniprot.org

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