| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CLU (314-449) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CLU |
| Gene ID: |
1191 |
| Uniprot ID: |
CLUS_HUMAN |
| Immunogen Region: |
314-449 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 314-449 of human Clusterin alpha chain (NP_001822.3). |
| Immunogen Sequence: |
STNNPSQAKLRRELDESLQV AERLTRKYNELLKSYQWKML NTSSLLEQLNEQFNWVSRLA NLTQGEDQYYLRVTTVASHT SDSDVPSGVTEVVVKLFDSD PITVTVPVEVSRKNPKFMET VAEKALQEYRKKHREE |
| Tissue Specificity | Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. |
| Post Translational Modifications | Proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Proteolytic cleavage is not necessary for its chaperone activity. All non-secreted forms are not proteolytically cleaved. Chaperone activity of uncleaved forms is dependent on a non-reducing environment. Polyubiquitinated, leading to proteasomal degradation. Under cellular stress, the intracellular level of cleaved form is reduced due to proteasomal degradation. Extensively glycosylated with sulfated N-linked carbohydrates. About 30% of the protein mass is comprised of complex N-linked carbohydrate. Endoplasmic reticulum (ER) stress induces changes in glycosylation status and increases level of hypoglycosylated forms. Core carbohydrates are essential for chaperone activity. Non-secreted forms are hypoglycosylated or unglycosylated. |
| Function | Isoform 1: Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury. Isoform 6: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Isoform 4: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Promotes cell death through interaction with BCL2L1 that releases and activates BAX. |
| Protein Name | ClusterinAging-Associated Gene 4 ProteinApolipoprotein JApo-JComplement Cytolysis InhibitorCliComplement-Associated Protein Sp-40 -40Ku70-Binding Protein 1Na1/Na2Sulfated Glycoprotein 2Sgp-2Testosterone-Repressed Prostate Message 2Trpm-2 Cleaved Into - Clusterin Beta ChainApojalphaComplement Cytolysis Inhibitor A ChainSp-40 -40 Beta-Chain - Clusterin Alpha ChainApojbetaComplement Cytolysis Inhibitor B ChainSp-40 -40 Alpha-Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-166665Reactome: R-HSA-6803157Reactome: R-HSA-977606 |
| Cellular Localisation | Isoform 1: SecretedCan Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular StressIsoform 4: CytoplasmKeeps Cytoplasmic Localization In Stressed And Unstressed CellIsoform 6: CytoplasmNucleusCytoplasmMitochondrion MembranePeripheral Membrane ProteinCytoplasmic SideCytosolMicrosomeEndoplasmic ReticulumMitochondrionPerinuclear RegionCytoplasmic VesicleSecretory VesicleChromaffin GranuleSecreted Isoforms Can Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular StressDetected In Perinuclear Foci That May Be Aggresomes Containing MisfoldedUbiquitinated ProteinsDetected At The Mitochondrion Membrane Upon Induction Of ApoptosisUnder Er StressA Immaturely Glycosylated Pre-Secreted Form Retrotranslocates From The Endoplasmic Reticulum (Er)-Golgi Network To The Cytoplasm To Localize In The Mitochondria Through Hspa5 InteractionEr Stress Reduces SecretionUnder The StressMinor Amounts Of Non-Secreted Forms Accumulate In CytoplasmNon-Secreted Forms Emerge Mainly From Failed TranslocationAlternative Splicing Or Non-Canonical Initiation Start Codon |
| Alternative Antibody Names | Anti-Clusterin antibodyAnti-Aging-Associated Gene 4 Protein antibodyAnti-Apolipoprotein J antibodyAnti-Apo-J antibodyAnti-Complement Cytolysis Inhibitor antibodyAnti-Cli antibodyAnti-Complement-Associated Protein Sp-40 -40 antibodyAnti-Ku70-Binding Protein 1 antibodyAnti-Na1/Na2 antibodyAnti-Sulfated Glycoprotein 2 antibodyAnti-Sgp-2 antibodyAnti-Testosterone-Repressed Prostate Message 2 antibodyAnti-Trpm-2 Cleaved Into - Clusterin Beta Chain antibodyAnti-Apojalpha antibodyAnti-Complement Cytolysis Inhibitor A Chain antibodyAnti-Sp-40 -40 Beta-Chain - Clusterin Alpha Chain antibodyAnti-Apojbeta antibodyAnti-Complement Cytolysis Inhibitor B Chain antibodyAnti-Sp-40 -40 Alpha-Chain antibodyAnti-CLU antibodyAnti-APOJ antibodyAnti-CLI antibodyAnti-KUB1 antibodyAnti-AAG4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
