• Western blot analysis of extracts of various cell lines, using CNOT2 antibody (STJ114698) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CNOT2 antibody (341-540) (STJ114698)

SKU:
STJ114698

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNOT2 (341-540) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNOT2
Gene ID: 4848
Uniprot ID: CNOT2_HUMAN
Immunogen Region: 341-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 341-540 of human CNOT2 (NP_055330.1).
Immunogen Sequence: GRVTNIPQGMVTDQFGMIGL LTFIRAAETDPGMVHLALGS DLTTLGLNLNSPENLYPKFA SPWASSPCRPQDIDFHVPSE YLTNIHIRDKLAAIKLGRYG EDLLFYLYYMNGGDVLQLLA AVELFNRDWRYHKEERVWIT RAPGMEPTMKTNTYERGTYY FFDCLNWRKVAKEFHLEYDK LEERPHLPSTFNYNPAQQAF
Tissue Specificity Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.
Function Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation.the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity.
Protein Name Ccr4-Not Transcription Complex Subunit 2
Ccr4-Associated Factor 2
Database Links Reactome: R-HSA-429947
Reactome: R-HSA-6804115
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Ccr4-Not Transcription Complex Subunit 2 antibody
Anti-Ccr4-Associated Factor 2 antibody
Anti-CNOT2 antibody
Anti-CDC36 antibody
Anti-NOT2 antibody
Anti-HSPC131 antibody
Anti-MSTP046 antibody

Information sourced from Uniprot.org

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