• Western blot analysis of extracts of various cell lines, using COL4A2 antibody (STJ29971) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COL4A2 antibody (1486-1712) (STJ29971)

SKU:
STJ29971

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL4A2 (1486-1712) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL4A2
Gene ID: 1284
Uniprot ID: CO4A2_HUMAN
Immunogen Region: 1486-1712
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1486-1712 of human COL4A2 (NP_001837.2).
Immunogen Sequence: VSIGYLLVKHSQTDQEPMCP VGMNKLWSGYSLLYFEGQEK AHNQDLGLAGSCLARFSTMP FLYCNPGDVCYYASRNDKSY WLSTTAPLPMMPVAEDEIKP YISRCSVCEAPAIAIAVHSQ DVSIPHCPAGWRSLWIGYSF LMHTAAGDEGGGQSLVSPGS CLEDFRATPFIECNGGRGTC HYYANKYSFWLTTIPEQSFQ GSPSADTLKAGLIRTHISRC QVCMKNL
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. Proteolytic processing produces the C-terminal NC1 peptide, canstatin.
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
Protein Name Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000157
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative Antibody Names Anti-Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin antibody
Anti-COL4A2 antibody

Information sourced from Uniprot.org

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