• Western blot analysis of extracts of various cell lines, using COQ6 antibody (STJ116547) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COQ6 antibody (169-468) (STJ116547)

SKU:
STJ116547

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COQ6 (169-468) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COQ6
Gene ID: 51004
Uniprot ID: COQ6_HUMAN
Immunogen Region: 169-468
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 169-468 of human COQ6 (NP_872282.1).
Immunogen Sequence: SKAIRYTWPCPFPMADSSPW VHITLGDGSTFQTKLLIGAD GHNSGVRQAVGIQNVSWNYD QSAVVATLHLSEATENNVAW QRFLPSGPIALLPLSDTLSS LVWSTSHEHAAELVSMDEEK FVDAVNSAFWSDADHTDFID TAGAMLQYAVSLLKPTKVSA RQLPPSVARVDAKSRVLFPL GLGHAAEYVRPRVALIGDAA HRVHPLAGQGVNMGFGDISS LAHHLSTAAFNGKDLGSVS
Tissue Specificity Widely expressed.
Function FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-hexaprenyl-4-hydroxybenzoic acid (HHB) to 3-hexaprenyl-4,5-dihydroxybenzoic acid (DHHB). The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6. Is able to perform the deamination reaction at C4 of 3-hexaprenyl-4-amino-5-hydroxybenzoic acid (HHAB) to produce DHHB when expressed in yeast cells lacking COQ9, even if utilization of para-aminobenzoic acid (pABA) involving C4-deamination seems not to occur in bacteria, plants and mammals, where only C5 hydroxylation of HHB has been shown.
Protein Name Ubiquinone Biosynthesis Monooxygenase Coq6 - Mitochondrial
Coenzyme Q10 Monooxygenase 6
Database Links Reactome: R-HSA-2142789
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Golgi Apparatus
Cell Projection
Localizes To Cell Processes And Golgi Apparatus In Podocytes
Alternative Antibody Names Anti-Ubiquinone Biosynthesis Monooxygenase Coq6 - Mitochondrial antibody
Anti-Coenzyme Q10 Monooxygenase 6 antibody
Anti-COQ6 antibody
Anti-CGI-10 antibody

Information sourced from Uniprot.org

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