• Western blot analysis of extracts of various cell lines, using CREM antibody (STJ27591) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CREM antibody (1-100) (STJ27591)

SKU:
STJ27591

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CREM (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CREM
Gene ID: 1390
Uniprot ID: CREM_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CREM (NP_853549.1).
Immunogen Sequence: MSKCARKKYIKTNPRQMTME TVESQHDGSITASLTESKSA HVQTQTGQNSIPALAQVAAI AETDESAESEGVIDSHKRRE ILSRRPSYRKILNELSSDVP
Tissue Specificity Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
Post Translational Modifications Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome. Stimulated by phosphorylation. Phosphorylated on Ser-116 by TSSK4 in vitro.
Function Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation. Isoform 6: May play a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.
Protein Name Camp-Responsive Element Modulator
Inducible Camp Early Repressor
Icer
Database Links Reactome: R-HSA-400253 Q03060-6
Cellular Localisation Nucleus
Isoform 6: Cytoplasm
Alternative Antibody Names Anti-Camp-Responsive Element Modulator antibody
Anti-Inducible Camp Early Repressor antibody
Anti-Icer antibody
Anti-CREM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance