• Western blot analysis of extracts of various cell lines, using CRH antibody (STJ23221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CRH antibody (25-194) (STJ23221)

SKU:
STJ23221

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CRH (25-194) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRH
Gene ID: 1392
Uniprot ID: CRF_HUMAN
Immunogen Region: 25-194
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-194 of human CRH (NP_000747.1).
Immunogen Sequence: LLSRGPVPGARQAPQHPQPL DFFQPPPQSEQPQQPQARPV LLRMGEEYFLRLGNLNKSPA APLSPASSLLAGGSGSRPSP EQATANFFRVLLQQLLLPRR SLDSPAALAERGARNALGGH QEAPERERRSEEPPISLDLT FHLLREVLEMARAEQLAQQA HSNRKLMEII
Tissue Specificity Produced by the hypothalamus and placenta.
Function Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
Protein Name Corticoliberin
Corticotropin-Releasing Factor
Crf
Corticotropin-Releasing Hormone
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-418555
Reactome: R-HSA-9022702
Cellular Localisation Secreted
Alternative Antibody Names Anti-Corticoliberin antibody
Anti-Corticotropin-Releasing Factor antibody
Anti-Crf antibody
Anti-Corticotropin-Releasing Hormone antibody
Anti-CRH antibody

Information sourced from Uniprot.org

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