• Western blot analysis of extracts of Rat brain, using CRMP1 antibody (STJ23226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of Neuro-2a cells using CRMP1 rabbit polyclonal antibody (STJ23226) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using CRMP1 rabbit polyclonal antibody (STJ23226) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-CRMP1 antibody (460-540) (STJ23226)

SKU:
STJ23226

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CRMP1 (460-540) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRMP1
Gene ID: 1400
Uniprot ID: DPYL1_HUMAN
Immunogen Region: 460-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-540 of human CRMP1 (NP_001304.1).
Immunogen Sequence: NVNKGMGRFIPRKAFPEHLY QRVKIRNKVFGLQGVSRGMY DGPVYEVPATPKYATPAPSA KSSPSKHQPPPIRNLHQSNF S
Tissue Specificity Brain.
Post Translational Modifications Phosphorylation at Ser-522 enhances CRMP1-mediated alteration of FLNA ternary structure and FLNA dissociation from F-actin.
Function Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance. During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone. Involved in invasive growth and cell migration. May participate in cytokinesis.
Protein Name Dihydropyrimidinase-Related Protein 1
Drp-1
Collapsin Response Mediator Protein 1
Crmp-1
Inactive Dihydropyrimidinase
Unc-33-Like Phosphoprotein 3
Ulip-3
Database Links Reactome: R-HSA-399956
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Cell Projection
Growth Cone
Perikaryon
Associated With Centrosomes And The Mitotic Spindle During Metaphase
Colocalizes With Flna And Tubulin In The Central Region Of Drg Neuron Growth Cone
Following Sema3a Stimulation Of Drg Neurons
Colocalizes With F-Actin
Alternative Antibody Names Anti-Dihydropyrimidinase-Related Protein 1 antibody
Anti-Drp-1 antibody
Anti-Collapsin Response Mediator Protein 1 antibody
Anti-Crmp-1 antibody
Anti-Inactive Dihydropyrimidinase antibody
Anti-Unc-33-Like Phosphoprotein 3 antibody
Anti-Ulip-3 antibody
Anti-CRMP1 antibody
Anti-DPYSL1 antibody
Anti-ULIP3 antibody

Information sourced from Uniprot.org

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