• Western blot analysis of extracts of A375 cells, using NG2/CSPG4 antibody (STJ29905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using NG2/CSPG4 antibody (STJ29905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts from normal (control) and NG2/CSPG4 knockout (KO) HeLa cells, using NG2/CSPG4 antibody (STJ29905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded mouse brain using NG2/CSPG4 antibody (STJ29905) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A-375 cells using [KO Validated] NG2/CSPG4 rabbit polyclonal antibody (STJ29905) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CSPG4 antibody (1340-1563) (STJ29905)

SKU:
STJ29905

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSPG4 (1340-1563) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSPG4
Gene ID: 1464
Uniprot ID: CSPG4_HUMAN
Immunogen Region: 1340-1563
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Immunogen Sequence: LGAPLEGVLVELEVLPAAIP LEAQNFSVPEGGSLTLAPPL LRVSGPYFPTLLGLSLQVLE PPQHGALQKEDGPQARTLSA FSWRMVEEQLIRYVHDGSET LTDSFVLMANASEMDRQSHP VAFTVTVLPVNDQPPILTTN TGLQMWEGATAPIPAEALRS TDGDSGSEDLVYTIEQPSNG RVVLRGAPGTEVRSFTQAQL DGGLVLFSHRGTLDGGFRFR LSDG
Tissue Specificity Detected in fibroblasts (at protein level). Detected in placenta (at protein level). Detected in malignant melanoma cells.
Post Translational Modifications O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Function Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Protein Name Chondroitin Sulfate Proteoglycan 4
Chondroitin Sulfate Proteoglycan Ng2
Melanoma Chondroitin Sulfate Proteoglycan
Melanoma-Associated Chondroitin Sulfate Proteoglycan
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022870
Reactome: R-HSA-2022923
Reactome: R-HSA-2024101
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3595172
Reactome: R-HSA-3595174
Reactome: R-HSA-3595177
Reactome: R-HSA-4420332
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Extracellular Side
Apical Cell Membrane
Cell Projection
Lamellipodium Membrane
Cell Surface
Localized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By Prkca
Localizes To The Retraction Fibers
Localizes To The Plasma Membrane Of Oligodendrocytes
Alternative Antibody Names Anti-Chondroitin Sulfate Proteoglycan 4 antibody
Anti-Chondroitin Sulfate Proteoglycan Ng2 antibody
Anti-Melanoma Chondroitin Sulfate Proteoglycan antibody
Anti-Melanoma-Associated Chondroitin Sulfate Proteoglycan antibody
Anti-CSPG4 antibody
Anti-MCSP antibody

Information sourced from Uniprot.org

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