• Western blot analysis of lysates from U-251MG cells, using DCAF4 Rabbit polyclonal antibody (STJ117069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DCAF4 antibody (1-120) (STJ117069)

SKU:
STJ117069

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCAF4 (1-120) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCAF4
Gene ID: 26094
Uniprot ID: DCAF4_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DCAF4 (NP_056419.2).
Immunogen Sequence: MNKSRWQSRRRHGRRSHQQN PWFRLRDSEDRSDSRAAQPA HDSGHGDDESPSTSSGTAGT SSVPELPGFYFDPEKKRYFR LLPGHNNCNPLTKESIRQKE MESKRLRLLQEEDRRKKIAR
Function May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Protein Name Ddb1- And Cul4-Associated Factor 4
Wd Repeat-Containing Protein 21a
Database Links Reactome: R-HSA-8951664
Alternative Antibody Names Anti-Ddb1- And Cul4-Associated Factor 4 antibody
Anti-Wd Repeat-Containing Protein 21a antibody
Anti-DCAF4 antibody
Anti-WDR21 antibody
Anti-WDR21A antibody

Information sourced from Uniprot.org

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