• Immunofluorescence analysis of U-2 OS cells using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DDX3Y in paraffin-embedded mouse heart using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DDX3Y in paraffin-embedded mouse brain using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DDX3Y in paraffin-embedded rat brain using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DDX3Y in paraffin-embedded rat testis using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DDX3Y Rabbit polyclonal antibody (STJ113303) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX3Y antibody (1-210) (STJ113303)

SKU:
STJ113303

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX3Y (1-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX3Y
Gene ID: 8653
Uniprot ID: DDX3Y_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
Immunogen Sequence: MSHVVVKNDPELDQQLANLD LNSEKQSGGASTASKGRYIP PHLRNREASKGFHDKDSSGW SCSKDKDAYSSFGSRDSRGK PGYFSERGSGSRGRFDDRGR SDYDGIGNRERPGFGRFERS GHSRWCDKSVEDDWSKPLPP SERLEQELFSGGNTGINFEK YDDIPVEATGSNCPPHIENF SDIDMGEIIMGNIELTRYTR PTPVQKHAIP
Tissue Specificity Widely expressed at the mRNA level, with highest levels in testis. Testis-specific (at protein level). Expressed predominantly in spermatogonia, but occasionally detected in some pre-leptotene/leptotene spermatocytes.
Function Probable ATP-dependent RNA helicase. During immune response, may enhance IFNB1 expression via IRF3/IRF7 pathway.
Protein Name Atp-Dependent Rna Helicase Ddx3y
Dead Box Protein 3 - Y-Chromosomal
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm In An Xpo1-Dependent Manner
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Ddx3y antibody
Anti-Dead Box Protein 3 - Y-Chromosomal antibody
Anti-DDX3Y antibody
Anti-DBY antibody

Information sourced from Uniprot.org

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