• Immunofluorescence analysis of SH-SY5Y cells using DLK1 Rabbit polyclonal antibody (STJ23392) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using DLK1 Rabbit polyclonal antibody (STJ23392) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-DLK1 antibody (24-303) (STJ23392)

SKU:
STJ23392

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DLK1 (24-303) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLK1
Gene ID: 8788
Uniprot ID: DLK1_HUMAN
Immunogen Region: 24-303
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-303 of human DLK1 (P80370).
Immunogen Sequence: AECFPACNPQNGFCEDDNVC RCQPGWQGPLCDQCVTSPGC LHGLCGEPGQCICTDGWDGE LCDRDVRACSSAPCANNRTC VSLDDGLYECSCAPGYSGKD CQKKDGPCVINGSPCQHGGT CVDDEGRASHASCLCPPGFS GNFCEIVANSCTPNPCENDG VCTDIGGDFRCRCPAGFIDK TCSRPVTNCASSPCQNGGTC LQHTQVSYECLCKPEFTGLT CVKKRALSPQQVTRLPSGY
Tissue Specificity Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
Post Translational Modifications N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function May have a role in neuroendocrine differentiation.
Protein Name Protein Delta Homolog 1
Dlk-1
Pg2 Cleaved Into - Fetal Antigen 1
Fa1
Database Links Reactome: R-HSA-2122948
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Protein Delta Homolog 1 antibody
Anti-Dlk-1 antibody
Anti-Pg2 Cleaved Into - Fetal Antigen 1 antibody
Anti-Fa1 antibody
Anti-DLK1 antibody
Anti-DLK antibody

Information sourced from Uniprot.org

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