• Western blot analysis of extracts of various cell lines, using DYNLL1 antibody (STJ116707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat ovary using DYNLL1 rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using DYNLL1 rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using DYNLL1 rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using DYNLL1 rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using DYNLL1 rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using DYNLL1 rabbit polyclonal antibody (STJ116707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DYNLL1 antibody (1-89) (STJ116707)

SKU:
STJ116707

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DYNLL1 (1-89) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DYNLL1
Gene ID: 8655
Uniprot ID: DYL1_HUMAN
Immunogen Region: 1-89
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-89 of human DYNLL1 (NP_003737.1).
Immunogen Sequence: MCDRKAVIKNADMSEEMQQD SVECATQALEKYNIEKDIAA HIKKEFDKKYNPTWHCIVGR NFGSYVTHETKHFIYFYLGQ VAILLFKSG
Tissue Specificity Ubiquitous. Expressed in testis.
Post Translational Modifications Phosphorylation at Ser-88 appears to control the dimer-monomer transition. it is phosphorylated at Ser-88 by PAK1, however, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.
Function Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1. Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase/NOS1.
Protein Name Dynein Light Chain 1 - Cytoplasmic
8 Kda Dynein Light Chain
Dlc8
Dynein Light Chain Lc8-Type 1
Protein Inhibitor Of Neuronal Nitric Oxide Synthase
Pin
Database Links Reactome: R-HSA-111446
Reactome: R-HSA-141444
Reactome: R-HSA-1632852
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-2565942
Reactome: R-HSA-3371497
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5620924
Reactome: R-HSA-5663220
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-68877
Reactome: R-HSA-8854518
Reactome: R-HSA-9609690
Reactome: R-HSA-9646399
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Mitochondrion
Upon Induction Of Apoptosis Translocates Together With Bcl2l11 To Mitochondria
Alternative Antibody Names Anti-Dynein Light Chain 1 - Cytoplasmic antibody
Anti-8 Kda Dynein Light Chain antibody
Anti-Dlc8 antibody
Anti-Dynein Light Chain Lc8-Type 1 antibody
Anti-Protein Inhibitor Of Neuronal Nitric Oxide Synthase antibody
Anti-Pin antibody
Anti-DYNLL1 antibody
Anti-DLC1 antibody
Anti-DNCL1 antibody
Anti-DNCLC1 antibody
Anti-HDLC1 antibody

Information sourced from Uniprot.org

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