Anti-E-cadherin antibody

[STJ92819] Download PDF Print Data Sheet

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Product name Anti-E-cadherin antibody
Short Description Rabbit polyclonal against E-cadherin
Description E-cadherin is a protein encoded by the CDH1 gene which is approximately 97,4 kDa. E-cadherin is localised to the cell membrane and Golgi apparatus. It is involved in cell junction organization, ERK signalling and apoptotic cleavage of cellular proteins. It is a calcium-dependent cell-cell adhesion protein that is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is also a ligand for integrin alpha-E/beta-7. E-cadherin is expressed in non-neural epithelial tissues. Mutations in the CDH1 gene may result in endometrial cancer and breast cancer. STJ92819 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of E-cadherin protein.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity E-cadherin Polyclonal Antibody detects endogenous levels of E-cadherin protein.
Protein Name Cadherin-1
CAM 120/80
Epithelial cadherin
E-cadherin
Uvomorulin
CD antigen CD324
E-Cad/CTF1
E-Cad/CTF2
E-Cad/CTF3
Immunogen Synthesized peptide derived from human E-cadherin
Immunogen Region 810-890 aa, C-terminal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 999
Gene Symbol CDH1
Molecular Weight 97 kDa
Database Links HGNC:1748
OMIM:119580
Alternative Names Cadherin-1
CAM 120/80
Epithelial cadherin
E-cadherin
Uvomorulin
CD antigen CD324
E-Cad/CTF1
E-Cad/CTF2
E-Cad/CTF3
Function Cadherins are calcium-dependent cell adhesion proteins . They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells . Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Post-translational Modifications During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.
Cellular Localization Cell junction Cell membrane. Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Tissue Specificity Non-neural epithelial tissues.
Sequence and Domain Family Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Swiss-Prot Key P12830_HUMAN
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