| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ECHS1 (28-290) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ECHS1 |
| Gene ID: |
1892 |
| Uniprot ID: |
ECHM_HUMAN |
| Immunogen Region: |
28-290 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-290 of human ECHS1 (NP_004083.3). |
| Immunogen Sequence: |
ASGANFEYIIAEKRGKNNTV GLIQLNRPKALNALCDGLID ELNQALKTFEEDPAVGAIVL TGGDKAFAAGADIKEMQNLS FQDCYSSKFLKHWDHLTQVK KPVIAAVNGYAFGGGCELAM MCDIIYAGEKAQFAQPEILI GTIPGAGGTQRLTRAVGKSL AMEMVLTGDRISAQDAKQAG LVSKICPVETLVEEAIQCAE KIASNSKIVVAMAKESVNAA FEMTLTEGSKLEKKLFYST |
| Tissue Specificity | Liver, fibroblast, muscle. Barely detectable in spleen and kidney. |
| Function | Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16. Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate. Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA). |
| Protein Name | Enoyl-Coa Hydratase - MitochondrialMechMech1Enoyl-Coa Hydratase 1Echs1Short-Chain Enoyl-Coa HydrataseSceh |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-77310Reactome: R-HSA-77346Reactome: R-HSA-77348Reactome: R-HSA-77350Reactome: R-HSA-77352 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Enoyl-Coa Hydratase - Mitochondrial antibodyAnti-Mech antibodyAnti-Mech1 antibodyAnti-Enoyl-Coa Hydratase 1 antibodyAnti-Echs1 antibodyAnti-Short-Chain Enoyl-Coa Hydratase antibodyAnti-Sceh antibodyAnti-ECHS1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
