• Western blot analysis of extracts of various cell lines, using EDNRB antibody (STJ23473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EDNRB antibody (1-100) (STJ23473)

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STJ23473

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDNRB (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDNRB
Gene ID: 1910
Uniprot ID: EDNRB_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human EDNRB (NP_001116131.1).
Immunogen Sequence: MQPPPSLCGRALVALVLACG LSRIWGEERGFPPDRATPLL QTAEIMTPPTKTLWPKGSNA SLARSLAPAEVPKGDRTAGS PPRTISPPPCQGPIEIKETF
Tissue Specificity Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
Post Translational Modifications Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
Function Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Protein Name Endothelin Receptor Type B
Et-B
Et-Br
Endothelin Receptor Non-Selective Type
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Internalized After Activation By Endothelins
Alternative Antibody Names Anti-Endothelin Receptor Type B antibody
Anti-Et-B antibody
Anti-Et-Br antibody
Anti-Endothelin Receptor Non-Selective Type antibody
Anti-EDNRB antibody
Anti-ETRB antibody

Information sourced from Uniprot.org

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