Anti-EGFR antibody

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Product name Anti-EGFR antibody
Short Description Rabbit monoclonal against EGFR
Description EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB- EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Applications IHC
Dilution range 1:50-100
Protein Name Epidermal growth factor receptor
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor
Storage Instruction Store at 2-8°C for up to 1 year
Note For Research Use Only (RUO).
Validated Application IHC
Host Rabbit
Clonality Monoclonal
Reactivity Human
Conjugation Unconjugated
Isotype IgG
Formulation Tissue culture supernatant with 0.2% BSA and 15mM sodium azide.
Gene ID 1956
Gene Symbol EGFR
Molecular Weight 134 kDa
Database Links HGNC:3236
OMIM:131550
Reactome:R-HSA-1227986
Alternative Names Epidermal growth factor receptor
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses, Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF, Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues, The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades, Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules, May also activate the NF-kappa-B signaling cascade, Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling, Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin, Plays a role in enhancing learning and memory performance (By similarity),
Post-translational Modifications Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated, Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation, Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane, Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6, Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3, Dephosphorylated by PTPN1 and PTPN2.
Cellular Localization Cell membrane
Tissue Specificity Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Swiss-Prot Key P00533
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