• Western blot analysis of extracts of various cell lines, using EHHADH antibody (STJ28146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EHHADH antibody (444-723) (STJ28146)

SKU:
STJ28146

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EHHADH (444-723) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHHADH
Gene ID: 1962
Uniprot ID: ECHP_HUMAN
Immunogen Region: 444-723
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 444-723 of human EHHADH (NP_001957.2).
Immunogen Sequence: EVIPSQYSSPTTIATVMNLS KKIKKIGVVVGNCFGFVGNR MLNPYYNQAYFLLEEGSKPE EVDQVLEEFGFKMGPFRVSD LAGLDVGWKSRKGQGLTGPT LLPGTPARKRGNRRYCPIPD VLCELGRFGQKTGKGWYQYD KPLGRIHKPDPWLSKFLSRY RKTHHIEPRTISQDEILERC LYSLINEAFRILGEGIAASP EHIDVVYLHGYGWPRHKGGP MFYASTVGLPTVLEKLQKY
Tissue Specificity Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.
Post Translational Modifications Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
Function Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway. Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways. Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation.
Protein Name Peroxisomal Bifunctional Enzyme
Pbe
Pbfe
L-Bifunctional Protein
Lbp
Multifunctional Enzyme 1
Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase
Database Links Reactome: R-HSA-390247
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Peroxisomal Bifunctional Enzyme antibody
Anti-Pbe antibody
Anti-Pbfe antibody
Anti-L-Bifunctional Protein antibody
Anti-Lbp antibody
Anti-Multifunctional Enzyme 1 antibody
Anti-Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase antibody
Anti-EHHADH antibody
Anti-ECHD antibody

Information sourced from Uniprot.org

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