• Western blot analysis of extracts of various cell lines, using EHMT1 antibody (STJ110811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EHMT1 antibody (1-260) (STJ110811)

SKU:
STJ110811

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EHMT1 (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHMT1
Gene ID: 79813
Uniprot ID: EHMT1_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EHMT1 (NP_079033.4).
Immunogen Sequence: MAAADAEAVPARGEPQQDCC VKTELLGEETPMAADEGSAE KQAGEAHMAADGETNGSCEN SDASSHANAAKHTQDSARVN PQDGTNTLTRIAENGVSERD SEAAKQNHVTADDFVQTSVI GSNGYILNKPALQAQPLRTT STLASSLPGHAAKTLPGGAG KGRTPSAFPQTPAAPPATLG EGSADTEDRKLPAPGADVKV HRARKTMPKSVVGLHAASKD PREVREARDHKEPKEEINK
Tissue Specificity Widely expressed.
Function Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B.
Protein Name Histone-Lysine N-Methyltransferase Ehmt1
Euchromatic Histone-Lysine N-Methyltransferase 1
Eu-Hmtase1
G9a-Like Protein 1
Glp
Glp1
Histone H3-K9 Methyltransferase 5
H3-K9-Hmtase 5
Lysine N-Methyltransferase 1d
Database Links Reactome: R-HSA-2559582
Reactome: R-HSA-3214841
Reactome: R-HSA-6804760
Reactome: R-HSA-8853884
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Chromosome
Associates With Euchromatic Regions
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Ehmt1 antibody
Anti-Euchromatic Histone-Lysine N-Methyltransferase 1 antibody
Anti-Eu-Hmtase1 antibody
Anti-G9a-Like Protein 1 antibody
Anti-Glp antibody
Anti-Glp1 antibody
Anti-Histone H3-K9 Methyltransferase 5 antibody
Anti-H3-K9-Hmtase 5 antibody
Anti-Lysine N-Methyltransferase 1d antibody
Anti-EHMT1 antibody
Anti-EUHMTASE1 antibody
Anti-GLP antibody
Anti-KIAA1876 antibody
Anti-KMT1D antibody

Information sourced from Uniprot.org

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