• Western blot analysis of extracts of various cell lines, using EIF1 antibody (STJ112681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF1 antibody (1-113) (STJ112681)

SKU:
STJ112681

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF1 (1-113) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF1
Gene ID: 10209
Uniprot ID: EIF1_HUMAN
Immunogen Region: 1-113
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human EIF1 (NP_005792.1).
Immunogen Sequence: MSAIQNLHSFDPFADASKGD DLLPAGTEDYIHIRIQQRNG RKTLTTVQGIADDYDKKKLV KAFKKKFACNGTVIEHPEYG EVIQLQGDQRKNICQFLVEI GLAKDDQLKVHGF
Function Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. Together with eIF1A (EIF1AX), EIF1 facilitates scanning and is essential for start codon recognition on the basis of AUG nucleotide context and location relative to the 5'-cap. Participates to initiation codon selection by influencing the conformation of the 40S ribosomal subunit and the positions of bound mRNA and initiator tRNA.this is possible after its binding to the interface surface of the platform of the 40S ribosomal subunit close to the P-site. Together with eIF1A (EIF1AX), also regulates the opening and closing of the mRNA binding channel, which ensures mRNA recruitment, scanning and the fidelity of initiation codon selection. Continuously monitors and protects against premature and partial base-pairing of codons in the 5'-UTR with the anticodon of initiator tRNA. Together with eIF1A (EIF1AX), acts for ribosomal scanning, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. Interacts with EIF4G1, which in a mutual exclusive interaction associates either with EIF1 or with EIF4E on a common binding site. EIF4G1-EIF1 complex promotes ribosome scanning (on both short and long 5'UTR), leaky scanning (on short 5'UTR) which is the bypass of the initial start codon, and discrimination against cap-proximal AUG. Is probably maintained within the 43S PIC in open conformation thanks to eIF1A-EIF5 interaction. Once the correct start codon is reached, EIF1 is physically excluded from the decoding site, shifting the PIC into the closed conformation and arresting it at the start codon.
Protein Name Eukaryotic Translation Initiation Factor 1
Eif1
A121
Protein Translation Factor Sui1 Homolog
Sui1iso1
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 1 antibody
Anti-Eif1 antibody
Anti-A121 antibody
Anti-Protein Translation Factor Sui1 Homolog antibody
Anti-Sui1iso1 antibody
Anti-EIF1 antibody
Anti-SUI1 antibody

Information sourced from Uniprot.org

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