• Immunofluorescence analysis of U-2 OS cells using ETHE1 Polyclonal Antibody (STJ112181) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ETHE1 antibody (STJ112181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ETHE1 antibody (STJ112181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ETHE1 antibody (STJ112181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using ETHE1 antibody (STJ112181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ETHE1 antibody (8-245) (STJ112181)

SKU:
STJ112181

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ETHE1 (8-245) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ETHE1
Gene ID: 23474
Uniprot ID: ETHE1_HUMAN
Immunogen Region: 8-245
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 8-245 of human ETHE1 (NP_055112.2).
Immunogen Sequence: VARRQLSQRGGSGAPILLRQ MFEPVSCTFTYLLGDRESRE AVLIDPVLETAPRDAQLIKE LGLRLLYAVNTHCHADHITG SGLLRSLLPGCQSVISRLSG AQADLHIEDGDSIRFGRFAL ETRASPGHTPGCVTFVLNDH SMAFTGDALLIRGCGRTDFQ QGCAKTLYHSVHEKIFTLPG DCLIYPAHDYHGFTVSTVEE ERTLNPRLTLSCEEFVKIMG NLNLPKPQQIDFAVPANM
Tissue Specificity Ubiquitously expressed.
Function Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. First described as a protein that can shuttle between the nucleus and the cytoplasm and suppress p53-induced apoptosis by sequestering the transcription factor RELA/NFKB3 in the cytoplasm and preventing its accumulation in the nucleus.
Protein Name Persulfide Dioxygenase Ethe1 - Mitochondrial
Ethylmalonic Encephalopathy Protein 1
Hepatoma Subtracted Clone One Protein
Sulfur Dioxygenase Ethe1
Database Links Reactome: R-HSA-1614517
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion Matrix
Alternative Antibody Names Anti-Persulfide Dioxygenase Ethe1 - Mitochondrial antibody
Anti-Ethylmalonic Encephalopathy Protein 1 antibody
Anti-Hepatoma Subtracted Clone One Protein antibody
Anti-Sulfur Dioxygenase Ethe1 antibody
Anti-ETHE1 antibody
Anti-HSCO antibody

Information sourced from Uniprot.org

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