• Western blot analysis of extracts of various cell lines, using EVPL antibody (STJ112253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EVPL antibody (1784-2033) (STJ112253)

SKU:
STJ112253

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EVPL (1784-2033) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EVPL
Gene ID: 2125
Uniprot ID: EVPL_HUMAN
Immunogen Region: 1784-2033
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1784-2033 of human EVPL (NP_001979.2).
Immunogen Sequence: TKPSSSLSIGSIISKSPLAS PAPQSTSFFSPSFSLGLGDD SFPIAGIYDTTTDNKCSIKT AVAKNMLDPITGQKLLEAQA ATGGIVDLLSRERYSVHKAM ERGLIENTSTQRLLNAQKAF TGIEDPVTKKRLSVGEAVQK GWMPRESVLPHLQVQHLTGG LIDPKRTGRIPIQQALLSGM ISEELAQLLQDESSYEKDLT DPISKERLSYKEAMGRCRKD PLSGLLLLPAALEGYRCYR
Tissue Specificity Exclusively expressed in stratified squamous epithelia.
Function Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
Protein Name Envoplakin
210 Kda Cornified Envelope Precursor Protein
210 Kda Paraneoplastic Pemphigus Antigen
P210
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cell Junction
Desmosome
Cornified Envelope
Cytoplasm
Cytoskeleton
Colocalized With Dsp At Desmosomes And Along Intermediate Filaments
Alternative Antibody Names Anti-Envoplakin antibody
Anti-210 Kda Cornified Envelope Precursor Protein antibody
Anti-210 Kda Paraneoplastic Pemphigus Antigen antibody
Anti-P210 antibody
Anti-EVPL antibody

Information sourced from Uniprot.org

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