| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EXT2 (80-340) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EXT2 |
| Gene ID: |
2132 |
| Uniprot ID: |
EXT2_HUMAN |
| Immunogen Region: |
80-340 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EXT2 (NP_000392.3). |
| Immunogen Sequence: |
PHSIESSNDWNVEKRSIRDV PVVRLPADSPIPERGDLSCR MHTCFDVYRCGFNPKNKIKV YIYALKKYVDDFGVSVSNTI SREYNELLMAISDSDYYTDD INRACLFVPSIDVLNQNTLR IKETAQAMAQLSRWDRGTNH LLFNMLPGGPPDYNTALDVP RDRALLAGGGFSTWTYRQGY DVSIPVYSPLSAEVDLPEKG PGPRQYFLLSSQVGLHPEYR EDLEALQVKHGESVLVLDK |
| Tissue Specificity | Ubiquitous. |
| Function | Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan. |
| Protein Name | Exostosin-2Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-AcetylglucosaminyltransferaseMultiple Exostoses Protein 2Putative Tumor Suppressor Protein Ext2 |
| Database Links | Reactome: R-HSA-2022928Reactome: R-HSA-3656237Reactome: R-HSA-3656253 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinGolgi Apparatus MembraneGolgi ApparatusCis-Golgi Network MembraneThe Ext1/Ext2 Complex Is Localized In The Golgi Apparatus |
| Alternative Antibody Names | Anti-Exostosin-2 antibodyAnti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibodyAnti-Multiple Exostoses Protein 2 antibodyAnti-Putative Tumor Suppressor Protein Ext2 antibodyAnti-EXT2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98784/382834/STJ119999_1__50326.1713151342.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
