| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FBXL2 (120-240) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FBXL2 |
| Gene ID: |
25827 |
| Uniprot ID: |
FBXL2_HUMAN |
| Immunogen Region: |
120-240 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human FBXL2 (NP_036289.3). |
| Immunogen Sequence: |
STCYSLSRFCSKLKHLDLTS CVSITNSSLKGISEGCRNLE YLNLSWCDQITKDGIEALVR GCRGLKALLLRGCTQLEDEA LKHIQNYCHELVSLNLQSCS RITDEGVVQICRGCHRLQAL C |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, pancreas and placenta. |
| Post Translational Modifications | Phosphorylated by GSK-beta (GSK3B), promoting recognition by FBXO3, leading to its ubiquitination by the SCF(FBXO3) complex. Ubiquitinated at Lys-201 by the SCF(FBXO3) complex in response to lipopolysaccharide (LPS), leading to its degradation by the proteasome. |
| Function | Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant. The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3. |
| Protein Name | F-Box/Lrr-Repeat Protein 2F-Box And Leucine-Rich Repeat Protein 2F-Box Protein Fbl2/Fbl3 |
| Cellular Localisation | MembraneLipid-Anchor |
| Alternative Antibody Names | Anti-F-Box/Lrr-Repeat Protein 2 antibodyAnti-F-Box And Leucine-Rich Repeat Protein 2 antibodyAnti-F-Box Protein Fbl2/Fbl3 antibodyAnti-FBXL2 antibodyAnti-FBL2 antibodyAnti-FBL3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
