• Immunofluorescence analysis of HeLa cells using FLNC Rabbit polyclonal antibody (STJ114985) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using FLNC Rabbit polyclonal antibody (STJ114985) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FLNC antibody (2160-2340) (STJ114985)

SKU:
STJ114985

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FLNC (2160-2340) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLNC
Gene ID: 2318
Uniprot ID: FLNC_HUMAN
Immunogen Region: 2160-2340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2160-2340 of human FLNC (NP_001449.3).
Immunogen Sequence: IPGNWFQMVSAQERLTRTFT RSSHTYTRTERTEISKTRGG ETKREVRVEESTQVGGDPFP AVFGDFLGRERLGSFGSITR QQEGEASSQDMTAQVTSPSG KVEAAEIVEGEDSAYSVRFV PQEMGPHTVAVKYRGQHVPG SPFQFTVGPLGEGGAHKVRA GGTGLERGVAGVPAEFSIWT R
Tissue Specificity Highly expressed in striated muscles. Weakly expressed in thyroid, fetal brain, fetal lung, retina, spinal cord and bone marrow. Not expressed in testis, pancreas, adrenal gland, placenta, liver and kidney.
Post Translational Modifications Ubiquitinated by FBXL22, leading to proteasomal degradation.
Function Muscle-specific filamin, which plays a central role in sarcomere assembly and organization. Critical for normal myogenesis, it probably functions as a large actin-cross-linking protein with structural functions at the Z lines in muscle cells. May be involved in reorganizing the actin cytoskeleton in response to signaling events.
Protein Name Filamin-C
Fln-C
Flnc
Abp-280-Like Protein
Abp-L
Actin-Binding-Like Protein
Filamin-2
Gamma-Filamin
Database Links Reactome: R-HSA-446353
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Cytoskeleton
Myofibril
Sarcomere
Z Line
A Small Amount Localizes At Membranes
In Striated Muscle Cells
It Predominantly Localizes In Myofibrillar Z Lines
While A Minor Fraction Localizes With Subsarcolemme
Targeting To Developing And Mature Z Lines Is Mediated By The Intradomain Insert
Alternative Antibody Names Anti-Filamin-C antibody
Anti-Fln-C antibody
Anti-Flnc antibody
Anti-Abp-280-Like Protein antibody
Anti-Abp-L antibody
Anti-Actin-Binding-Like Protein antibody
Anti-Filamin-2 antibody
Anti-Gamma-Filamin antibody
Anti-FLNC antibody
Anti-ABPL antibody
Anti-FLN2 antibody

Information sourced from Uniprot.org

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