• Western blot analysis of extracts of various cell lines, using GABARAPL1 antibody (STJ110100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GABARAPL1 antibody (1-50) (STJ110100)

SKU:
STJ110100

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GABARAPL1 (1-50) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABARAPL1
Gene ID: 23710
Uniprot ID: GBRL1_HUMAN
Immunogen Region: 1-50
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-50 of human GABARAPL1 (NP_113600.1).
Immunogen Sequence: MKFQYKEDHPFEYRKKEGEK IRKKYPDRVPVIVEKAPKAR VPDLDKRKYL
Tissue Specificity Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
Post Translational Modifications The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAPL1-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAPL1-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms required for GABARAPL1 recycling when autophagosomes fuse with lysosomes. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS).
Function Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
Protein Name Gamma-Aminobutyric Acid Receptor-Associated Protein-Like 1
Early Estrogen-Regulated Protein
Gaba(A Receptor-Associated Protein-Like 1
Glandular Epithelial Cell Protein 1
Gec-1
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasmic Vesicle
Autophagosome
Cytoplasmic Vesicle Membrane
Lipid-Anchor
Cytoplasm
Cytoskeleton
Endoplasmic Reticulum
Golgi Apparatus
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor-Associated Protein-Like 1 antibody
Anti-Early Estrogen-Regulated Protein antibody
Anti-Gaba(A Receptor-Associated Protein-Like 1 antibody
Anti-Glandular Epithelial Cell Protein 1 antibody
Anti-Gec-1 antibody
Anti-GABARAPL1 antibody
Anti-GEC1 antibody

Information sourced from Uniprot.org

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