• Western blot analysis of extracts of various cell lines, using GABRG2 antibody (STJ23734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-GABRG2 antibody (400-500) (STJ23734)

SKU:
STJ23734

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GABRG2 (400-500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABRG2
Gene ID: 2566
Uniprot ID: GBRG2_HUMAN
Immunogen Region: 400-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human GABRG2 (NP_944493.2).
Immunogen Sequence: VSNRKPSKDKDKKKKNPLLR MFSFKAPTIDIRPRSATIQM NNATHLQERDEEYGYECLDG KDCASFFCCFEDCRTGAWRH GRIHIRIAKMDSYARIFFPT A
Post Translational Modifications Glycosylated. Palmitoylated by ZDHHC3/GODZ.required for the accumulation of GABA(A) receptors at the postsynaptic membrane of inhibitory GABAergic synapses.
Function Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer. The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity. The alpha2/beta2/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity. Functions also as histamine receptor and mediates cellular responses to histamine.
Protein Name Gamma-Aminobutyric Acid Receptor Subunit Gamma-2
Gaba(A Receptor Subunit Gamma-2
Database Links Reactome: R-HSA-1236394
Reactome: R-HSA-977443
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cell Projection
Dendrite
Cytoplasmic Vesicle Membrane
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor Subunit Gamma-2 antibody
Anti-Gaba(A Receptor Subunit Gamma-2 antibody
Anti-GABRG2 antibody

Information sourced from Uniprot.org

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