• Western blot analysis of extracts of various cell lines, using GADD45A antibody (STJ113355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using GADD45A antibody (STJ113355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded rat lung using GADD45A antibody (STJ113355) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using GADD45A antibody (STJ113355) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using GADD45A antibody (STJ113355) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of U2OS cells using GADD45A antibody (STJ113355) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GADD45A antibody (1-165) (STJ113355)

SKU:
STJ113355

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GADD45A (1-165) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GADD45A
Gene ID: 1647
Uniprot ID: GA45A_HUMAN
Immunogen Region: 1-165
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1).
Immunogen Sequence: MTLEEFSAGEQKTERMDKVG DALEEVLSKALSQRTITVGV YEAAKLLNVDPDNVVLCLLA ADEDDDRDVALQIHFTLIQA FCCENDINILRVSNPGRLAE LLLLETDAGPAASEGAEQPP DLHCVLVTNPHSSQWKDPAL SQLICFCRESRYMDQWVPVI NLPER
Function In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes.stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
Protein Name Growth Arrest And Dna Damage-Inducible Protein Gadd45 Alpha
Dna Damage-Inducible Transcript 1 Protein
Ddit-1
Database Links Reactome: R-HSA-6804114
Reactome: R-HSA-9617828
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd45 Alpha antibody
Anti-Dna Damage-Inducible Transcript 1 Protein antibody
Anti-Ddit-1 antibody
Anti-GADD45A antibody
Anti-DDIT1 antibody
Anti-GADD45 antibody

Information sourced from Uniprot.org

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