• Western blot analysis of extracts of various cell lines, using GAPDHS antibody (STJ112498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GAPDHS antibody (179-408) (STJ112498)

SKU:
STJ112498

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GAPDHS (179-408) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GAPDHS
Gene ID: 26330
Uniprot ID: G3PT_HUMAN
Immunogen Region: 179-408
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 179-408 of human GAPDHS (NP_055179.1).
Immunogen Sequence: ASDHISAGAQRVVISAPSPD APMFVMGVNENDYNPGSMNI VSNASCTTNCLAPLAKVIHE RFGIVEGLMTTVHSYTATQK TVDGPSRKAWRDGRGAHQNI IPASTGAAKAVTKVIPELKG KLTGMAFRVPTPDVSVVDLT CRLAQPAPYSAIKEAVKAAA KGPMAGILAYTEDEVVSTDF LGDTHSSIFDAKAGIALNDN FVKLISWYDNEYGYSHRVVD LLRYMFSRDK
Tissue Specificity Testis specific.
Function May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
Protein Name Glyceraldehyde-3-Phosphate Dehydrogenase - Testis-Specific
Spermatogenic Cell-Specific Glyceraldehyde 3-Phosphate Dehydrogenase 2
Gapdh-2
Spermatogenic Glyceraldehyde-3-Phosphate Dehydrogenase
Database Links Reactome: R-HSA-390471
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Glyceraldehyde-3-Phosphate Dehydrogenase - Testis-Specific antibody
Anti-Spermatogenic Cell-Specific Glyceraldehyde 3-Phosphate Dehydrogenase 2 antibody
Anti-Gapdh-2 antibody
Anti-Spermatogenic Glyceraldehyde-3-Phosphate Dehydrogenase antibody
Anti-GAPDHS antibody
Anti-GAPD2 antibody
Anti-GAPDH2 antibody
Anti-GAPDS antibody
Anti-HSD-35 antibody
Anti-HSD35 antibody

Information sourced from Uniprot.org

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