• Immunofluorescence analysis of PC-12 cells using GCLM Rabbit polyclonal antibody (STJ27267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GCLM antibody (STJ27267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GCLM antibody (1-274) (STJ27267)

SKU:
STJ27267

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GCLM (1-274) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCLM
Gene ID: 2730
Uniprot ID: GSH0_HUMAN
Immunogen Region: 1-274
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
Immunogen Sequence: MGTDSRAAKALLARARTLHL QTGNLLNWGRLRKKCPSTHS EELHDCIQKTLNEWSSQINP DLVREFPDVLECTVSHAVEK INPDEREEMKVSAKLFIVES NSSSSTRSAVDMACSVLGVA QLDSVIIASPPIEDGVNLSL EHLQPYWEELENLVQSKKIV AIGTSDLDKTQLEQLYQWAQ VKPNSNQVNLASCCVMPPDL TAFAKQFDIQLLTHNDPKEL LSEASFQEALQESIPDIQA
Tissue Specificity In all tissues examined. Highest levels in skeletal muscle.
Protein Name Glutamate--Cysteine Ligase Regulatory Subunit
Gcs Light Chain
Gamma-Ecs Regulatory Subunit
Gamma-Glutamylcysteine Synthetase Regulatory Subunit
Glutamate--Cysteine Ligase Modifier Subunit
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-5578999
Reactome: R-HSA-9818027
Alternative Antibody Names Anti-Glutamate--Cysteine Ligase Regulatory Subunit antibody
Anti-Gcs Light Chain antibody
Anti-Gamma-Ecs Regulatory Subunit antibody
Anti-Gamma-Glutamylcysteine Synthetase Regulatory Subunit antibody
Anti-Glutamate--Cysteine Ligase Modifier Subunit antibody
Anti-GCLM antibody
Anti-GLCLR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance