• Western blot analysis of extracts of various cell lines, using GJB1 antibody (STJ112151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GJB1 antibody (214-283) (STJ112151)

SKU:
STJ112151

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GJB1 (214-283) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GJB1
Gene ID: 2705
Uniprot ID: CXB1_HUMAN
Immunogen Region: 214-283
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 214-283 of human GJB1 (NP_000157.1).
Immunogen Sequence: IRACARRAQRRSNPPSRKGS GFGHRLSPEYKQNEINKLLS EQDGSLKDILRRSPGTGAGL AEKSDRCSAC
Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Protein Name Gap Junction Beta-1 Protein
Connexin-32
Cx32
Gap Junction 28 Kda Liver Protein
Database Links Reactome: R-HSA-190704
Reactome: R-HSA-190827
Reactome: R-HSA-190861
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Alternative Antibody Names Anti-Gap Junction Beta-1 Protein antibody
Anti-Connexin-32 antibody
Anti-Cx32 antibody
Anti-Gap Junction 28 Kda Liver Protein antibody
Anti-GJB1 antibody
Anti-CX32 antibody

Information sourced from Uniprot.org

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