• Western blot analysis of extracts of various cell lines, using GJC1 antibody (STJ29133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GJC1 antibody (247-396) (STJ29133)

SKU:
STJ29133

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GJC1 (247-396) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GJC1
Gene ID: 10052
Uniprot ID: CXG1_HUMAN
Immunogen Region: 247-396
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 247-396 of human GJC1 (NP_005488.2).
Immunogen Sequence: IWEMLHLGFGTIRDSLNSKR RELEDPGAYNYPFTWNTPSA PPGYNIAVKPDQIQYTELSN AKIAYKQNKANTAQEQQYGS HEENLPADLEALQREIRMAQ ERLDLAVQAYSHQNNPHGPR EKKAKVGSKAGSNKSTASSK SGDGKTSVWI
Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Protein Name Gap Junction Gamma-1 Protein
Connexin-45
Cx45
Gap Junction Alpha-7 Protein
Database Links Reactome: R-HSA-112303
Reactome: R-HSA-190861
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Alternative Antibody Names Anti-Gap Junction Gamma-1 Protein antibody
Anti-Connexin-45 antibody
Anti-Cx45 antibody
Anti-Gap Junction Alpha-7 Protein antibody
Anti-GJC1 antibody
Anti-GJA7 antibody

Information sourced from Uniprot.org

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