• Immunohistochemistry analysis of paraffin-embedded rat bone marrow using GP9 Rabbit polyclonal antibody (STJ27327) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of K-562 cells, using GP9 antibody (STJ27327) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-GP9 antibody (17-147) (STJ27327)

SKU:
STJ27327

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GP9 (17-147) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GP9
Gene ID: 2815
Uniprot ID: GPIX_HUMAN
Immunogen Region: 17-147
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-147 of human GP9 (NP_000165.1).
Immunogen Sequence: TKDCPSPCTCRALETMGLWV DCRGHGLTALPALPARTRHL LLANNSLQSVPPGAFDHLPQ LQTLDVTQNPWHCDCSLTYL RLWLEDRTPEALLQVRCASP SLAAHGPLGRLTGYQLGSCG WQLQASWVRPG
Function The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
Protein Name Platelet Glycoprotein Ix
Gp-Ix
Gpix
Glycoprotein 9
Cd Antigen Cd42a
Database Links Reactome: R-HSA-140837
Reactome: R-HSA-430116
Reactome: R-HSA-75892
Reactome: R-HSA-76009
Reactome: R-HSA-9673221
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Platelet Glycoprotein Ix antibody
Anti-Gp-Ix antibody
Anti-Gpix antibody
Anti-Glycoprotein 9 antibody
Anti-Cd Antigen Cd42a antibody
Anti-GP9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance