• Western blot analysis of extracts of various cell lines, using GPC4 antibody (STJ114671) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GPC4 antibody (357-556) (STJ114671)

SKU:
STJ114671

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPC4 (357-556) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPC4
Gene ID: 2239
Uniprot ID: GPC4_HUMAN
Immunogen Region: 357-556
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 357-556 of human GPC4 (NP_001439.2).
Immunogen Sequence: SESAFSARFRPHHPEERPTT AAGTSLDRLVTDVKEKLKQA KKFWSSLPSNVCNDERMAAG NGNEDDCWNGKGKSRYLFAV TGNGLANQGNNPEVQVDTSK PDILILRQIMALRVMTSKMK NAYNGNDVDFFDISDESSGE GSGSGCEYQQCPSEFDYNAT DHAGKSANEKADSAGVRPGA QAYLLTVFCILFLVMQREWR
Function Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
Protein Name Glypican-4
K-Glypican Cleaved Into - Secreted Glypican-4
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-4420332
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Secreted Glypican-4: Secreted
Extracellular Space
Alternative Antibody Names Anti-Glypican-4 antibody
Anti-K-Glypican Cleaved Into - Secreted Glypican-4 antibody
Anti-GPC4 antibody
Anti-UNQ474 antibody
Anti-PRO937 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance