• Western blot analysis of extracts of mouse heart cells, using GPER1 antibody (STJ112255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody (STJ112255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U20S cells using GPER1 antibody (STJ112255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GPER1 antibody (STJ112255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GPER1 antibody (307-375) (STJ112255)

SKU:
STJ112255

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPER1 (307-375) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPER1
Gene ID: 2852
Uniprot ID: GPER1_HUMAN
Immunogen Region: 307-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-375 of human GPER1 (NP_001496.1).
Immunogen Sequence: HIVNLAAFSNSCLNPLIYSF LGETFRDKLRLYIEQKTNLP ALNRFCHAALKAVIPDSTEQ SDVRFSSAV
Tissue Specificity Expressed in placenta, endothelial and epithelial cells, non laboring and laboring term myometrium, fibroblasts and cancer-associated fibroblasts (CAF), prostate cancer cells and invasive adenocarcinoma (at protein level). Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
Post Translational Modifications Ubiquitinated.ubiquitination occurs at the plasma membrane and leads to proteasome-mediated degradation. Glycosylated.
Function G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.
Protein Name G-Protein Coupled Estrogen Receptor 1
Chemoattractant Receptor-Like 2
Flow-Induced Endothelial G-Protein Coupled Receptor 1
Feg-1
G Protein-Coupled Estrogen Receptor 1
G-Protein Coupled Receptor 30
Gpcr-Br
Il8-Related Receptor Dry12
Lymphocyte-Derived G-Protein Coupled Receptor
Lygpr
Membrane Estrogen Receptor
Mer
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-9634597
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Cytoskeleton
Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cytoplasmic Vesicle Membrane
Early Endosome
Recycling Endosome
Golgi Apparatus Membrane
Golgi Apparatus
Trans-Golgi Network
Endoplasmic Reticulum Membrane
Cell Projection
Dendrite
Dendritic Spine Membrane
Axon
Postsynaptic Density
Mitochondrion Membrane
Colocalized With Bsn To The Active Zone Of Presynaptic Density
Colocalized With Dlg4/Psd95 And Neurabin-2 Ppp1r9b In Neuronal Synaptosomes
Endocytosed In A Agonist- And Arrestin-Independent Manner
Colocalized With Ramp3 And Clathrin-Coated Pits At The Plasma Membrane
Colocalized With Transferrin Receptor At The Plasma Membrane And Perinuclear Region
Accumulated And Colocalized With Rab11 Proteins In Recycling Endosomes And Trans-Golgi Network (Tgn)
But Does Neither Recycle Back To The Cell Surface Nor Traffics To Late Endosome Or Lysosome
Colocalized With Calnexin In The Endoplasmic Reticulum
Traffics To Intracellular Sites Via Cytokeratin Intermediate Filaments Like Krt7 And Krt8 After Constitutive Endocytosis In Epithelial Cells
Colocalized With Egfr In The Nucleus Of Agonist-Induced Cancer-Associated Fibroblasts (Caf)
Alternative Antibody Names Anti-G-Protein Coupled Estrogen Receptor 1 antibody
Anti-Chemoattractant Receptor-Like 2 antibody
Anti-Flow-Induced Endothelial G-Protein Coupled Receptor 1 antibody
Anti-Feg-1 antibody
Anti-G Protein-Coupled Estrogen Receptor 1 antibody
Anti-G-Protein Coupled Receptor 30 antibody
Anti-Gpcr-Br antibody
Anti-Il8-Related Receptor Dry12 antibody
Anti-Lymphocyte-Derived G-Protein Coupled Receptor antibody
Anti-Lygpr antibody
Anti-Membrane Estrogen Receptor antibody
Anti-Mer antibody
Anti-GPER1 antibody
Anti-CEPR antibody
Anti-CMKRL2 antibody
Anti-DRY12 antibody
Anti-GPER antibody
Anti-GPR30 antibody

Information sourced from Uniprot.org

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