• Western blot analysis of SK-MEL-28, using GPNMB antibody (STJ116483) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of Rat eye, using GPNMB antibody (STJ116483) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GPNMB antibody (23-572) (STJ116483)

SKU:
STJ116483

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPNMB (23-572) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPNMB
Gene ID: 10457
Uniprot ID: GPNMB_HUMAN
Immunogen Region: 23-572
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-572 of human GPNMB (NP_001005340.1).
Immunogen Sequence: KRFHDVLGNERPSAYMREHN QLNGWSSDENDWNEKLYPVW KRGDMRWKNSWKGGRVQAVL TSDSPALVGSNITFAVNLIF PRCQKEDANGNIVYEKNCRN EAGLSADPYVYNWTAWSEDS DGENGTGQSHHNVFPDGKPF PHHPGWRRWNFIYVFHTLGQ YFQKLGRCSVRVSVNTANVT LGPQLMEVTVYRRHGRAYVP IAQVKDVYVVTDQIPVFVTM FQKNDRNSSDETFLKDLPI
Tissue Specificity Widely expressed, but very low expression, if any, in the brain. Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level). Expressed in peripheral blood, but not bone marrow mononuclear cells. Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme.
Function Could be a melanogenic enzyme.
Protein Name Transmembrane Glycoprotein Nmb
Hematopoietic Growth Factor Inducible Neurokinin-1 Type
Database Links Reactome: R-HSA-8857538
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Melanosome Membrane
Early Endosome Membrane
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Transmembrane Glycoprotein Nmb antibody
Anti-Hematopoietic Growth Factor Inducible Neurokinin-1 Type antibody
Anti-GPNMB antibody
Anti-HGFIN antibody
Anti-NMB antibody
Anti-UNQ1725 antibody
Anti-PRO9925 antibody

Information sourced from Uniprot.org

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