• Western blot analysis of Rat brain, using NMDAR1 antibody (STJ29990) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of mouse brain, using NMDAR1 antibody (STJ29990) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using NMDAR1 rabbit polyclonal antibody (STJ29990) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using NMDAR1 rabbit polyclonal antibody (STJ29990) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Neuro-2a cells using NMDAR1 rabbit polyclonal antibody (STJ29990) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GRIN1 antibody (810-910) (STJ29990)

SKU:
STJ29990

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRIN1 (810-910) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRIN1
Gene ID: 2902
Uniprot ID: NMDZ1_HUMAN
Immunogen Region: 810-910
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 810-910 of human NMDAR1 (NP_015566.1).
Immunogen Sequence: FENMAGVFMLVAGGIVAGIF LIFIEIAYKRHKDARRKQMQ LAFAAVNVWRKNLQDRKSGR AEPDPKKKATFRAITSTLAS SFKRRRSSKDTSTGGGRGAL Q
Post Translational Modifications NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
Protein Name Glutamate Receptor Ionotropic - Nmda 1
Glun1
Glutamate Nmda Receptor Subunit Zeta-1
N-Methyl-D-Aspartate Receptor Subunit Nr1
Nmd-R1
Database Links Reactome: R-HSA-3928662
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-6794361
Reactome: R-HSA-8849932
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Postsynaptic Density
Enriched In Postsynaptic Plasma Membrane And Postsynaptic Densities
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Nmda 1 antibody
Anti-Glun1 antibody
Anti-Glutamate Nmda Receptor Subunit Zeta-1 antibody
Anti-N-Methyl-D-Aspartate Receptor Subunit Nr1 antibody
Anti-Nmd-R1 antibody
Anti-GRIN1 antibody
Anti-NMDAR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance