• Western blot analysis of extracts of various cell lines, using GRPR antibody (STJ23875) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-GRPR antibody (235-384) (STJ23875)

SKU:
STJ23875

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRPR (235-384) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRPR
Gene ID: 2925
Uniprot ID: GRPR_HUMAN
Immunogen Region: 235-384
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 235-384 of human GRPR (NP_005305.1).
Immunogen Sequence: KNLIQSAYNLPVEGNIHVKK QIESRKRLAKTVLVFVGLFA FCWLPNHVIYLYRSYHYSEV DTSMLHFVTSICARLLAFTN SCVNPFALYLLSKSFRKQFN TQLLCCQPGLIIRSHSTGRS TTCMTSLKSTNPSVATFSLI NGNICHERYV
Tissue Specificity Highly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain. In brain, expressed in cells throughout the cortex.
Function Receptor for gastrin-releasing peptide (GRP). Signals via association with G proteins that activate a phosphatidylinositol-calcium second messenger system, resulting in Akt phosphorylation. Contributes to the regulation of food intake. Contributes to the perception of prurient stimuli and transmission of itch signals in the spinal cord that promote scratching behavior, but does not play a role in the perception of pain. Contributes primarily to nonhistaminergic itch sensation. In one study, shown to act in the amygdala as part of an inhibitory network which inhibits memory specifically related to learned fear. In another study, shown to contribute to disinhibition of glutamatergic cells in the auditory cortex via signaling on vasoactive intestinal peptide-expressing cells which leads to enhanced auditory fear memories. Contributes to the induction of sighing through signaling in the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity.
Protein Name Gastrin-Releasing Peptide Receptor
Grp-R
Grp-Preferring Bombesin Receptor
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Gastrin-Releasing Peptide Receptor antibody
Anti-Grp-R antibody
Anti-Grp-Preferring Bombesin Receptor antibody
Anti-GRPR antibody

Information sourced from Uniprot.org

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