• Western blot analysis of extracts of Raji cells, using HBB antibody (STJ111074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-HBB antibody (47-147) (STJ111074)

SKU:
STJ111074

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HBB (47-147) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HBB
Gene ID: 3043
Uniprot ID: HBB_HUMAN
Immunogen Region: 47-147
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 47-147 of human HBB (NP_000509.1).
Immunogen Sequence: GDLSTPDAVMGNPKVKAHGK KVLGAFSDGLAHLDNLKGTF ATLSELHCDKLHVDPENFRL LGNVLVCVLAHHFGKEFTPP VQAAYQKVVAGVANALAHKY H
Tissue Specificity Red blood cells.
Post Translational Modifications Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus. S-nitrosylated.a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2). Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure.
Function Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin: Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Protein Name Hemoglobin Subunit Beta
Beta-Globin
Hemoglobin Beta Chain Cleaved Into - Lvv-Hemorphin-7 - Spinorphin
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-2168880
Reactome: R-HSA-6798695
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Reactome: R-HSA-983231
Alternative Antibody Names Anti-Hemoglobin Subunit Beta antibody
Anti-Beta-Globin antibody
Anti-Hemoglobin Beta Chain Cleaved Into - Lvv-Hemorphin-7 - Spinorphin antibody
Anti-HBB antibody

Information sourced from Uniprot.org

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