• Western blot analysis of extracts of various cell lines, using HOOK1 antibody (STJ26831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HOOK1 antibody (489-728) (STJ26831)

SKU:
STJ26831

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HOOK1 (489-728) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HOOK1
Gene ID: 51361
Uniprot ID: HOOK1_HUMAN
Immunogen Region: 489-728
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 489-728 of human HOOK1 (NP_056972.1).
Immunogen Sequence: IEELQEQLEQKHRKMNELET EQRLSKERIRELQQQIEDLQ KSLQEQGSKSEGESSSKLKQ KLEAHMEKLTEVHEELQKKQ ELIEDLQPDINQNVQKINEL EAALQKKDEDMKAMEERYKM YLEKARNVIKTLDPKLNPAS AEIMLLRKQLAEKERRIEIL ESECKVAKFRDYEEKLIVSA WYNKSLAFQKLGMESRLVSG GGACSDTGACTPARSFLAQQ RHITNTRRNLSVKVPATTS
Function Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell. Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton.
Protein Name Protein Hook Homolog 1
H-Hook1
Hhk1
Cellular Localisation Cytoplasm
Cytoskeleton
Localizes To Punctate Cytoplasmic Foci Which Do Not Appear To Overlap With Early Or Late Endosomes
The Endoplasmic Reticulum
Multivesicular Bodies (Mvbs)
Lysosomes
Or Mitochondria
Often Found In Close Association With Microtubules
Does Not Associate With The Golgi Complex
During Spermiogenesis
It Localizes To The Manchette In Spermatids From Steps 8-10
It Is Also Present Between The Microtubule Manchette And The Nucleus
During Manchette Elongation
It Is Preferentially Localized To The Nuclear Ring Of The Manchette
Whereas The Strong Localization To The Manchette Decreases
In More Mature Spermatids
While The Manchette Migrates Posteriorly
It Localizes To Punctuates Spots
At Later Stages Of Spermatid Differentiation
The Punctuate Expression Pattern Is Found At Both The Attachment Site And The Proximal End Of The Elongated Manchette
In Contrast
It Is Not Present In Mature Spermatozoa
Alternative Antibody Names Anti-Protein Hook Homolog 1 antibody
Anti-H-Hook1 antibody
Anti-Hhk1 antibody
Anti-HOOK1 antibody

Information sourced from Uniprot.org

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