• Western blot analysis of extracts of various cell lines, using HTRA4 antibody (STJ116526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HTRA4 antibody (32-200) (STJ116526)

SKU:
STJ116526

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HTRA4 (32-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTRA4
Gene ID: 203100
Uniprot ID: HTRA4_HUMAN
Immunogen Region: 32-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-200 of human HTRA4 (NP_710159.1).
Immunogen Sequence: EKLHTQPSCPAVCQPTRCPA LPTCALGTTPVFDLCRCCRV CPAAEREVCGGAQGQPCAPG LQCLQPLRPGFPSTCGCPTL GGAVCGSDRRTYPSMCALRA ENRAARRLGKVPAVPVQWGN CGDTGTRSAGPLRRNYNFIA AVVEKVAPSVVHVQLWGRLL HGSRLVPVY
Function Serine protease.
Protein Name Serine Protease Htra4
High-Temperature Requirement Factor A4
Cellular Localisation Secreted
Alternative Antibody Names Anti-Serine Protease Htra4 antibody
Anti-High-Temperature Requirement Factor A4 antibody
Anti-HTRA4 antibody

Information sourced from Uniprot.org

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