• Western blot analysis of extracts of various cell lines, using IFI16 antibody (STJ24124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of U2OS cells using IFI16 antibody (STJ24124). Blue: DAPI for nuclear staining.

Anti-IFI16 antibody (1-785) (STJ24124)

SKU:
STJ24124

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFI16 (1-785) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFI16
Gene ID: 3428
Uniprot ID: IF16_HUMAN
Immunogen Region: 1-785
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-785 of human IFI16 (NP_001351796.1).
Immunogen Sequence: MGKKYKNIVLLKGLEVINDY HFRMVKSLLSNDLKLNLKMR EEYDKIQIADLMEEKFRGDA GLGKLIKIFEDIPTLEDLAE TLKKEKLKVKGPALSRKRKK EVDATSPAPSTSSTVKTEGA EATPGAQKRKKSTKEKAGPK GSKVSEEQTQPPSPAGAGMS TAMGRSPSPKTSLSAPPNSS STENPKTVAKCQVTPRRNVL QKRPVIVKVLSTTKPFEYET PEMEKKIMFHATVATQTQF
Tissue Specificity Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast (at protein level). Isoform IFI16-beta: Widely expressed.
Post Translational Modifications Ubiquitinated by human herpes simplex virus 1 (HHV-1) ICP0 protein.leading to degradation by the proteasome. Lysine acetylation in the multipartite nuclear localization signal (NLS) regulates the subcellular location. In vitro can be acetylated by p300/EP300 coupled to cytoplasmic localization. Phosphorylated on Ser and Thr. Isoform 3 seems to show a minor degree of complex carbohydrate addition.
Function Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Isoform IFI16-beta: Isoform that specifically inhibits the AIM2 inflammasome. Binds double-stranded DNA (dsDNA) in the cytoplasm, impeding its detection by AIM2. Also prevents the interaction between AIM2 and PYCARD/ASC via its interaction with AIM2, thereby inhibiting assembly of the AIM2 inflammasome. This isoform also weakly induce production of type I interferon-beta (IFNB1) via its interaction with STING1.
Protein Name Gamma-Interferon-Inducible Protein 16
Ifi-16
Interferon-Inducible Myeloid Differentiation Transcriptional Activator
Database Links Reactome: R-HSA-1834941
Reactome: R-HSA-3270619
Cellular Localisation Nucleus
Cytoplasm
Cellular Distribution Is Dependent On The Acetylation Status Of The Multipartite Nuclear Localization Signal (Nls)
Nls Acetylation Promotes Cytoplasmic Localization
Localizes In The Nucleus During Human Herpes Simplex Virus 1 (Hhv-1) Infection
Isoform Ifi16-Beta: Cytoplasm
Alternative Antibody Names Anti-Gamma-Interferon-Inducible Protein 16 antibody
Anti-Ifi-16 antibody
Anti-Interferon-Inducible Myeloid Differentiation Transcriptional Activator antibody
Anti-IFI16 antibody
Anti-IFNGIP1 antibody

Information sourced from Uniprot.org

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